Composite

Part:BBa_K3606035:Design

Designed by: Zhenru Zhang   Group: iGEM20_Fudan   (2020-10-23)


PhoA-CaAP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 138
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The composite part PhoA_CaAP is a combination of signal peptide PhoA and CaAP(Calcium Absorption Peptide). The sequence of PhoA is obtained from BBa_K808028 with no further codon optimization. Then we fused PhoA (BBa_K808028) to the N-terminal of CaAP(BBa_K3606000) for its secretion into the extracellular space.


Source

The sequence of PhoA is obtained from BBa_K808028 while the sequence of CAAP is artificially designed based on oligopeptides obtained from tilapia bone collagen hydrolysate, etc.

References